Biodegradation of Indigo Carmine Dye by Laccase from Bacillus licheniformis NS2324

Indigo carmine dye is used in many industries like textile, paper, cosmetics, etc. It specially textile for dyeing denim. The untreated water discharged from these industries, especially the industry, poses a significant environmental threat. conventional physicochemical methods treating industrial effluent are very expensive and cause secondary pollution. In present study, an extracellular laccase-producing Bacillus licheniformis NS2324 was isolated, crude enzyme to degrade indigo dye. conditions needed decolorisation were standardised. Optimisation studies by laccase revealed that able decolorize efficiently at 50 oC temperature, pH 8, with dose of 10 IU/ml after 6 hours. Up 96.79±1.06 per cent achieved under optimised without use any mediator laccase. Complete degradation absence makes study useful denim industry.